Summary: The group B vitamins have a wide variety of functions in the human body playing a crucial roles as a coenzymes for enzymatic reactions in the different tissues. Paricularly, vitamin B1 (thiamine), vitamin B6 (pyridoxine), and B12 (cobalamine) have a strong neurotropic effect. They provide energy to the nerve cells necessary for synthesis of DNA/RNA, neurotransmitters, and myelin. In the paper the review of the current knowledge of the usefulness of group B vitamins in CNS diseases, and a case report of female patient with neuropathic pain due to lumbo-sacral radiculopathy treated with combined formula of benfothiamine (B1 analogue), pyridoxine (B6 ) and cyanocobalamin (B12) were also presented.