Summary: Cholestasis is characterized by clinical and biochemical symptoms resulting from impaired bile production, its secretion into the bile canaliculi or its transport from the liver to the duodenum. Due to practical reasons cholestasis is divided into intrahepatic and extrahepatic. Differential diagnosis of cholestasis includes precisely collected medical history, laboratory results and imaging tests. Increase of activities of alkaline phosphatase (ALP) and gamma gluthamylotranspeptydase (GGTP), higher bilirubin and biliary acids levels are biochemical parameters of cholestasis. 1.5-fold increase in ALP activity and a 3-fold increase in GGTP activity are indicated to start diagnose of cholestasis. Abdominal USG is a primary imaging technique that allows to differentiate intra- from extraheaptic cholestasis. Dilatation of extrahepatic biliary ducts indicates extrahepatic cholestasis. The next procedure used in the diagnosis of cholestasis that is currently considered the gold standard is magnetic resonance cholangiopancreatography (MRCP). In cases where a cause of intrahepatic cholestasis cannot be established on the base of on noninvasive examinations, liver biopsy is necessary.